PerCP/Cy5.5 anti-human CD45RO

Antibodies Single
Sony
UCHL1
Flow Cytometry
Mouse IgG2a, κ
Human
IL-2 dependent T cell line, CA1
2121110
$376.00

Description

CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

References

1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (FC)
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5. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immunol. 5:430. (IHC)
6. Baba N, et al. 2010. Int. Immunol. 22:237. PubMed
7. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
8. Weiss L, et al. 2010. P. Natl. Acad. Sci. USA 107:10632. PubMed
9. Wu YY, et al. 2007. Infect. Immun. 75:4357. PubMed
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13. Smith SH, et al. 1986. Immunology 58:63. (Immunogen)