Purified anti-human HLA-G

Antibodies Single
Sony
87G
Other
Mouse IgG2a, κ
Human
HLA-G transfected cells
100 µg
2279510
$121.00

Description

HLA-G is a nonclassical MHC class I (MHC-Ib) molecule structurally related to MHC class Ia (HLA-A,B, C). There are seven isoforms of this molecule, including membrane bound HLA-G1, -G2, -G3 and -G4 and soluble HLA-G5, -G6, and -G7. HLA-G is primarily expressed on trophoblast cells. Its expression is also found on thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, and in various tumors. HLA-G exerts its inhibitory functions to regulate immune tolerance via interaction with inhibitory receptors ILT2(CD85j), ILT4(CD85d), and KIR2DL4(CD158d), which is expressed on NK cells, T cells, monocytes, dendritic cells, and B cells. HLA-G is also able to bind CD8α/α, which may mediate positive and/or negative selection in thymus.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 microg per 106 cells in 100 microL volume or 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Odum N, et al. 1991. Eur. J. Immunol. 21:2121.
2. Lila N, et al. 2001. P. Natl. Acad. Sci. USA 98:12150.
3. Lila N, et al. 2002. Circulation. 105:1949.
4. Blaschitz A, et al. 2000. Hum. Immunol. 61:1074.
5. Balsamo M, et al. 2012. Eur J. Immunol. 42:1833. PubMed