Alexa Fluor® 488 anti-Histone H3-Phosphorylated (Ser28)

Antibodies Single
Sony
HTA28
Flow Cytometry
Rat IgG2a, κ
Human
Synthetic peptide conjugated to KLH, corresponding to amino acids 23-35 of human histone H3.
25 tests
3805015
$139.00

Description

H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication and chromosomal stability. During mitosis, H3 is phosphorylated at serine 28.This phosphorylation coincides with chromosome condensation initiated at prophase and disappears at late anaphase. H3 has been demonstrated to be phosphorylated by the action of MLTK-α (mixed linage kinase-like mitogen activated protein triple kinase α) in response to ultraviolet B light and epidermal growth factor, as well as Aurora-B during mitosis.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. Please follow theCell Fixation and Permeabilization Protocol Using 70% Ethanol. For flow cytometric staining, the suggested use of this reagent is 5 microL per million cells or 5 microL per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

References

1. Hirata A, et al. 2004. J. Histochem. Cytochem. 52:1503.
2. Goto H, et al. 1999. J. Biol. Chem. 274:25543.
3. Ozawa K. 2008. Cytometry A 73:517.
3. Goode NJ, et al. 2014. PLoS Genet. 10:1004323. PubMed