Alexa Fluor® 647 anti-mouse TCR Vβ11

Antibodies Single
Sony
KT11
Flow Cytometry
Rat IgG2b, κ
Mouse
100 µg
1229545
$260.00

Description

The Vβ11 gene locus is deleted in mice of TCR a haplotype (C57BR, C57L, SJL, SWR) and TCR c haplotype (RIII).Mice of TCR b haplotype (C57BL, BALB/c, AKR, etc ) have Vβ11-bearing T cells .However, mice expressing MHC class II I-E and some superantigens, such as Mtv-8, Mtv-9 and Mtv-11 will show a partial deletion of Tb11-bearing T cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

References

1. Quigley MF, et al. 2010. Proc Natl Acad Sci USA. 9:19414. PubMed