The B20.6 antibody recognizes V β 2 TCR of all strains tested. V β 2 bearing T lymphocytes are clonally eliminated in MHC class II I-E expressing mice neonatally infected with MMTV virus through milk feeding.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.
References
1. Necker A, et al. 1991. Eur J. Immunol. 21:3035 2. Hodes RJ, et al. 1993. J Immunol 150:1422