Alexa Fluor® 647 anti-mouse CD73
Description
CD73 (ecto-5`-nucleotidase) is a 69 kD GPI-anchored surface protein. In mice, expression of CD73 in bone marrow is restricted to CD11b+ myeloid cells. In spleen, it is largely expressed on T cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 microg per 106 cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
References
1. Yamshita Y, et al 1998. Eur. J. Immunol. 28:2981.
2. Bentley JK, et al. 2010. Respir. Res. 11:127. (IHC-paraffin)
3. Kaku H, et al. 2014. J Immunol. 193:5904. PubMed