Description

Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
2. Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
3. Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
4. Kayama H, et al. 2012. PNAS. 109:5010. PubMed
5. Park S, et al. 2013. Biomaterials. 34:598. PubMed
6. Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
7. McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed
8. Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed