Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 microg per million cells in 100 microL volume. For magnetic cell separation with MojoSort™ streptavidin Nanobeads (Cat. No. 480015/480016), the suggested use of this reagent is ≤20 microg per 10 million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.