APC anti-mouse CD25

Antibodies Single
Sony
3C7
Flow Cytometry
Rat IgG2b, κ
Mouse
IL-2-dependent BALB/c mouse helper T-cell clone HT-2
1109550
$290.00

Description

CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 microg per 106 cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Ortega RG, et al. 1984. J. Immunol. 133:1970. (Block)
2. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
3. Mushaben EM, et al. 2011. J. Immunol. 187:5756. PubMed
4. Rosalia RA, et al. 2013. Immunobiology. 218:851. PubMed