Description

The LN3 monoclonal antibody reacts with the HLA-DR antigen, a member of MHC class II molecules. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4⁺ T cells.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 microg per 10⁶ cells in 100 microL volume or 100 microL whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Marder RJ, et al. 1985. Lab. Invest. 52:497.
2. Norton AJ and Isaacson PG. 1987. Am. J. Pathol. 128:225.
3. Hua ZX, et al. 1998. Hum. Pathol. 29(12):1441.