PE anti-human CD172a/b (SIRPα/β)

Antibodies Single
Sony
SE5A5
Flow Cytometry
Mouse IgG1, κ
Human
NIH-3T3/hu-SIRPα cell line
2219030
$255.00

Description

CD172a, also known as signal-regulatory protein α (SIRPα), src homology 2 domain-containing phosphatase substrate-1 (SHPS1), PTPNS1, BIT, MFR, and P84, is a 75-110 kD transmembrane glycoprotein involved in receptor tyrosine kinase coupled signaling pathway. It belongs to the Ig superfamily and is primarily expressed on monocytes/macrophages, granulocytes, dendritic cells, and neurons. CD172a serves as a substrate of activated receptor tyrosine kinases (RTKs). The interaction of CD172a intracellular domain with SHP-1 and SHP-2 displays negative signaling in the regulation of leukocyte adhesion and transmigration, T cell activation, macrophage fusion, and phagocytosis. CD47 (IAP) is the extracellular ligand for CD172a. SIRPα was recently demonstrated to be a specifc marker for cardiomyocytes derived from human pluripotent stem cells2.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 microL to 5 microL per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 microL staining volume or per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Seiffert M, et al. 1999. Blood 94:3633.
2. Dubois NC, et al. 2011. Nat. Biotechnol. 29:1011.
3. Barros MM, et al. 2009. Transfusion 49:154.
4. Liu Y, et al. 2005. J. Biol. Chem. 280:36132.
5. Barclay AN. 2009. Curr. Opin. Immunol. 21:47.
6. Florian S, et al. 2005. J. Leukoc. Biol. 77:984.