APC anti-human Perforin
Description
Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 microL to 5 microL per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 microL staining volume or per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
References
1. Hameed A, et al. 1992. Am. J. Pathol. 140:1025. (IHC)
2. Schaerli P, et al. 2004. J. Exp. Med. 199:1265.
3. Watanabe N, et al. 1997. Blood 90:3662.
4. Mauad T, et al. 2004. Pediatr. Pulmonol. 38:233. (IHC)
5. Barrat FJ, et al. 1999. P. Natl. Acad. Sci. USA 96:8645. (IF)
6. Chen H, et al. 2005. J. Immunol. 175:591.
7. Bryceson YT, et al. 2007. Blood doi:10.1182/blood-2007-02-074468. (IF)
8. Wood SM, et al. 2009. Blood 114:4117. PubMed
9. Makedonas G, et al. 2010. PLoS Pathog. 6:e1000798.