Description

Ly-6A/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses. The E13-161.7 antibody has been reported to be useful for immunohistochemical staining of frozen sections, immunoprecipitation, flow cytometry, and complement-mediated cytotoxicity.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD)
2. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC)
3. van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP)
4. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF)
5. Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed
6. Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed
7. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
8. Zheng Z, et al. 2012. Biochem Biophys Res Commun. 428:309. PubMed