Alexa Fluor® 488 anti-rat CD45

Antibodies Single
Sony
OX-1
Immunofluorescence
Mouse IgG1, κ
Rat
Enriched glycoprotein fraction from Wistar rat thymocytes
100 µg
1611050
$226.00

Description

CD45 is a 180-220 kD protein also known as leukocyte common antigen (LCA). It is a protein tyrosine phosphatase with multiple isoforms differing as a result of alternative splicing of the extracellular domain and glycosylation. CD45 is expressed on all hematopoietic cells except erythrocytes and platelets; isoform expression depends on cell type, activation state, and cell maturation. CD45 functions in signal transduction through T and B cell antigen receptors. CD45 has been shown to interact with various proteins including galectin-1, CD2, CD3, and CD4. The OX-1 antibody has been shown to partially inhibit NK cell-mediated lysis of syngeneic tumor cells in vitro.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

References

1. Giezeman-Smits KM, et al. 1999. J. Immunol. 163:71. (IP)
2. Elbe A, et al. 1994. J. Invest. Dermatol. 102:74. (IF)
3. Kouwenhoven E, et al. 2001. Kidney Int. 59:1142. (IHC)
4. Martin A, et al. 1995 Clin. Exp. Immunol. 22:283. (IHC)
5. Sayegh MH, et al. 1995 J. Exp. Med. 181:186. (IHC)
6. Morioka Y, et al. 2000. Kidney Int. 60:2192. (IHC)
7. Ng YY, et al. 2005. Kidney Int. 94:S83. (IHC-P)
8. Huang XR, et al. 2003. JASN. 14:1738. (IHC-P)