Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2^b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.

References

1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
2. Viville S, et al. 1993. Cell 72:635. (IHC)
3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
6. Guo M, et al. 1995. Zygote 3:65. (IHC)
7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed