Description

CD49d is a 150 kD glycoprotein, also known as α₄ integrin or VLA-4 α chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells. CD49d is a heterodimer expressed with either of two β chains, β1 (CD29) or β7, to form the VLA-4 (integrin α₄β₁) or LPAM-1 (integrin α₄β₇) complexes. CD49d plays a critical role in adhesion and T cell costimulation. The primary ligands for CD49d are VCAM-1, MAdCAM-1, and fibronectin.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

References

1. Holzmann B, et al. 1989. Cell 56:37. (IP, Block)
2. Ferguson TA, et al. 1993. J. Immunol. 150:1172. (Block)
3. Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
4. Kilshaw PJ, et al. 1991. Eur. J. Immunol. 21:2591. (Block)
5. Jaspers M, et al. 1995. Differentiation 59:79. (IHC)
6. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)