Alexa Fluor® 647 anti-mouse Ly-51
Description
Ly-51 is a 140 kD protein also known as 6C3/BP-1. It is a homodimeric cell-surface glycoprotein with aminopeptidase A (APA) activity. Ly-51 is expressed on B cell progenitors, bone marrow stromal cell lines, thymic dendritic cells, and cortical epithelial cells. Ly-51 expression can be upregulated by IL-7 stimulation.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
References
1. Pillemer E, et al. 1984. P. Natl. Acad. Sci. USA 81:4434. (IP)
2. Hardy RR, et al. 1991. J. Exp. Med. 173:1213. (IP)
3. Adkins B, et al. 1988. Immunogenetics 27:180. (IHC)
4. Surh CD, et al. 1992. J. Exp. Med. 176:611. (IHC)
5. Goverman J, et al. 1997. Immunol. Today 18:204. (IHC)
6. Dumont-Lagace M, 2014. J. Immunol. 192:2219. PubMed
7. Kvell K, et al. 2010. PLoS One 5:e10701. (FC, IHC)
8. Griewank K, et al. 2007. Immunity 27:751. (FC, IHC)