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- Sony Biotechnology
The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kb haplotype. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
1. Loken MR, et al. 1982. J. Immunol. Methods 50:R85.
2. Wall KA, et al. 1983. J. Immunol. 131:1056.
3. Andersson M, et al. 1998. J. Immunol. 161:6475.
4. Shao H, et al. 2005. J. Immunol. 175:1851.
5. Hui S, et al. 2005. J. Immunol. 175:1851. PubMed
6. Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed
7. Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed
8. Martin-Granados C, 2015. J Mol Cell Biol. PubMed