Alexa Fluor® 647 anti-mouse CD23
Description
CD23 is a 45 kD protein also known as low affinity IgE Fc receptor, FcεRII, BLAST-2, Ly-42, or B6. It is a member of the Ig family, expressed on conventional B (but not B-1) cells and follicular dendritic cells. CD23 responds to high levels of IgE by downregulating IgE secretion.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 microg per million cells in 100 microL volume. For immunofluorescence microscopy, a concentration range of 2.5-10 microg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
References
1. Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP)
2. Rao M, et al. 1987. J. Immunol. 138:1845. (Block)
3. Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block)
4. Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block)
5. Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC)
6. Craig VJ, et al. 2011. Cancer Res. 71:3616. PubMed