Description

NRF2 is a transcription factor that plays a critical role in inducing expression of genes required for oxidative stress defense and stress balance. It does so by binding to antioxidant response elements (AREs), which are located upstream of target genes. NRF2 is degraded by ubiquitination of KEAP1 E3 ligase. In a clinical setting, NRF2 is frequently activated in many types of cancers; it drives metabolic adaptation and survival in ROS-rich tumor microenvironments.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. For immunohistochemistry, a concentration range of 5.0 - 20.0 µg/mL is suggested. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP)
2. Rao M, et al. 1987. J. Immunol. 138:1845. (Block)
3. Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block)
4. Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block)
5. Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC)
6. Craig VJ, et al. 2011. Cancer Res. 71:3616. PubMed