Description

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is a ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

References

1. Hathcock KS, et al. 1993. Science 262:905. (Block, IP)
2. Inaba KM, et al. 1994. J. Exp. Med. 180:1849. (Block, IHC)
3. Hathcock KS, et al. 1994. J. Exp. Med. 180:631. (Block)
4. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block)
5. Liu Y, et al. 1997. J. Exp. Med. 185:251. (Block)
6. Herold KC, et al. 1997. J. Immunol. 158:984. (Block, IHC)
7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
8. Lawson BR, et al. 2007. J. Immunol. 178:5366.
9. Turnquist HR, et al. 2007. J. Immunol. 178:7018.
10. Klinger MB, et al. 2007. Am. J. Physiol. Requl. Integr. Comp. Physiol. 293:R677. PubMed
11. Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed