PE/Cyanine7 anti-mouse F4/80 Recombinant

Antibodies Single
Sony
QA17A29
Flow Cytometry
Mouse IgG1, κ
Mouse
Murine macrophages
1386540
$318.00

Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

  1. Sacco P, et al. 1995. J. Biol. Chem. 270:20201. (WB)
  2. Johnson KR, et al. 1993. Exp. Cell Res. 207:252.
  3. Gupta K, et al. 2012. J. Ped. Hem. Onc. 34:320. (IHC-P)
  4. Radice G, et al. 1997. Dev. Bio. 181:64. (IHC-P)