CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.
References
Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
Olver S, et al. 2006. Cancer Research 66:571. (FC)
Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
Fazilleau N, et al. 2007. Nature Immunol. 8:753.
Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed