Alexa Fluor® 647 anti-mouse CD25

Antibodies Single
Sony
PC61
Immunofluorescence
Rat IgG1, λ
Mouse
IL-2-dependent cytolytic mouse T-cell clone B6.1
1110095
$98.00

Description

CD25 is a 55 kD glycoprotein also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subsets, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132 (common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 microg per 106 cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for other applications.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

References

1. Lowenthal JW, et al. 1985. Nature 315:669. (IP, Block)
2. Ceredig R, et al. 1985. Nature 314:98. (IP, IHC, Block)
3. Lowenthal JW, et al. 1985. J. Immunol. 135:3988. (Block)
4. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
5. Takahashi T, et al. 2000. J. Exp. Med. 192:303. (Deplete)
6. Onizuka S, et al. 1999. Cancer Res. 59:3128. (Deplete)
7. Lei TC, et al. 2005. Blood 105:4865. (Deplete)
8. Pasare C, et al. 2004. Immunity 21:733. (Deplete)
9. León-Ponte M, et al. 2007. Blood 109:3139.
10. Cao OW, et al. 2007. Blood doi:10.1182/blood-2007-02-073304. (Deplete)
11. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719.