Description

The KH95 antibody reacts with the H-2D^b MHC class I alloantigen expressed on nucleated cells from mice of the H-2D^b haplotype. H-2D^b is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. Reactivity with other haplotypes (e.g., a,d,f,k,n,p,q,r,s,u,v) has not been reported.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

References

1. Hasenkrug KJ, et al. 1987. Immunogenetics 25:136.
2. Shao H, et al. 2005. J. Immunol. 175:1851.
3. Ponomarev ED, et al. 2006. J. Immunol. 176:1402.
4. Robb RJ, et al. 2012 Blood. 119:5898. PubMed
5. Zhang P, et al. 2013. J. Immunol. 191:5291. PubMed
6. Quinn KM, et al. 2013. J. Immunol. 191:5085. PubMed
7. Markey KA, et al. 2014. J Immunol. 192:5426. PubMed
8. Hogan T, et al. 2014. PLoS Comput Biol. 10:1003805. PubMed