PE/Fire™ 700 anti-mouse CD3

Antibodies Single
Sony
17A2
Flow Cytometry
Rat IgG2b, κ
Mouse
γδTCR-positive T-T hybridoma D1
1101360
$363.00

Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 700 has a maximum excitation of 565 nm and a maximum emission of 695 nm.

References

  1. Andersson J, et al. 1994. Immunology 83:16. (IHC)
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  3. Abrams JS. 1995. Curr. Prot. Immunol. Unit 6.20.
  4. Fernandez V, et al. 1994. Eur. J. Immunol. 24:1808. (IHC)
  5. Skansen-Saphir U, et al. 1994. Eur. J. Immunol. 24:916. (IHC)
  6. Andersson U, et al. Detection and Quantification of Gene Expression. New York:Springer-Verlag. (IHC)
  7. Prussin C, et al. 1995. J. Immunol. Methods. 188:117.
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  11. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
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  15. Maksaereekul S, et al. 2009. Vaccine. 28:3754 (FC) PubMed