CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Fire™ 700 has a maximum excitation of 565 nm and a maximum emission of 695 nm.
References
Andersson J, et al. 1994. Immunology 83:16. (IHC)
Abrams J, et al. 1992. Immunol. Rev. 127:5. (IP)
Abrams JS. 1995. Curr. Prot. Immunol. Unit 6.20.
Fernandez V, et al. 1994. Eur. J. Immunol. 24:1808. (IHC)
Skansen-Saphir U, et al. 1994. Eur. J. Immunol. 24:916. (IHC)
Andersson U, et al.Detection and Quantification of Gene Expression. New York:Springer-Verlag. (IHC)
Prussin C, et al. 1995. J. Immunol. Methods. 188:117.
Raqib R, et al. 2002. Infect. Immun. 70:3199. (IHC)
Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
Colleton BA, et al. 2009. J Virol. 83:6288. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)