APC/Fire™ 750 anti-human CD40

Antibodies Single
Sony
HB14
Flow Cytometry
Mouse IgG1, κ
Human,Non-human primate,Other
Ag8.653 myeloma cells
2165105
$152.00

Description

CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The HB14 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially block CD40 binding to CD40L and rescue B cells from apoptosis.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA)

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

References

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  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
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  4. Cavanagh LL, et al. 2005. Arthritis Res. Ther. 7:R230.
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  6. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  7. Iwata Y, et al. 2011. Blood. 117:530. PubMed