Purified anti-human IgM
Description
IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on immature and mature B cells, while IgM heavy (μ) chain is expressed intracellularly in pre-B cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 microg per 106 in 100 microL staining volume. It is recommended that the reagent be titrated for optimal performance for each application.
References
1. Polek TC, et al. 2003. J. Biol. Chem. 278:32317.
2. Nakayama M, et al. 2003. J. Immunol. 170:341.
3. Karaca G, et al. 2014. PLoS One. 9:83987. (IHC, IF, WB) PubMed
4. Muñoz-García B, et al. 2006. Stroke. 37:2044. (IF, WB)
5. Jakubowski A, et al. 2005. J. Clin. Invest. 115:2955. (IHC)