APC/Fire™ 750 anti-human CD223 (LAG-3)

Antibodies Single
Sony
11C3C65
Flow Cytometry
Mouse IgG1, κ
Human
Human LAG-3 transfected cells.
2446645
$164.00

Description

CD223, also known as LAG-3, is a 70 kD type I transmembrane glycoprotein that is involved in T-cell signaling. Similar to CD4, CD223 binds MHC class II, but with a higher affinity. CD223 negatively regulates T-cell activation. It is expressed by activated T-cells and natural killer cells (NKs), as well as regulatory T-cells. It is transiently expressed on the surface of activated T-cells in acute conditions but high expression is maintained under tolerizing conditions. CD223 deficiency results in reduced tumor growth. CD223 and PD-1 can act in synergy and reverse exhausted phenotypes, improve tumor rejection, and control viral load.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

References

  1. Bradstock KF, et al. 1980. J. Natl. Cancer Inst. 65:33.
  2. Csiba A, et al. 1984. Br. J. Cancer 50:699.
  3. Tchilian EZ, et al. 2001. J. Immunol. 166:1308.
  4. Lee MS, et al. 2004. Int. Immunol. 16:1109.