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  • APC/Fire™ 750 anti-mouse IFN-γ

APC/Fire™ 750 anti-mouse IFN-γ

APC/Fire™ 750 anti-mouse IFN-γ
Antibodies Single
Sony
XMG1.2
Intracellular Staining for Flow Cytometry
Rat IgG1, κ
Mouse
<I>E. coli</I>-expressed, recombinant mouse IFN-γ
3129295
$146.00
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Description

IFN-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

References

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  2. Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA, Neut)
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  4. Yang X, et al. 1993. J. Immunoassay 14:129. (ELISA)
  5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. (ELISPOT)
  6. Sander B, et al. 1991. Immunol. Rev. 119:65. (IHC)
  7. Ferrick D, et al. 1995. Nature 373:255. (FC)
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  16. Haring JS, et al. 2008. J. Immunol. 180:2855. (FC) PubMed
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  22. Yokogawa M, et al. 2013. Mol. Carcinog. 52:760. (IHC)
  23. Mottram PL, et al. 1998. J Immunol. 161:602. (IHC)