APC/Fire™ 750 anti-mouse CD366 (Tim-3)

Antibodies Single
Sony
RMT3-23
Flow Cytometry
Rat IgG2a, κ
Mouse
1198685
$182.00

Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

References

  1. Nakae S, et al. 2007. Blood 110(7):2565-8. (FC, Block)
  2. Oikawa T, et al. 2006. J. Immunol. 177(7):4281-7. (FC, Block, IHC)