CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.
References
Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
Pascal V, et al.2007. J. Immunol. 179:1751. PubMed