Description

Streptavidin binds to biotin with high affinity. Streptavidin-APC/Fire™ 750 is useful for detecting biotinylated antibodies. The excitation of APC/Fire™ 750 by 600-635 nm laser light induces a fluorescence emission maximum of 787 nm.

Formulation

Recommended Usage

Each lot of this Streptavidin APC/Fire™ 750 is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the APC/Fire™ 750 conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤0.25 µg per 10⁶ cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

*APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

References

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