Description

HMOX1 is a member of the heme oxygenase family. Unlike its constitutive counterpart, it is an inducible enzyme triggered by external stimuli such as nitric oxide, growth factors, heavy metals, UV radiation, phorbol esters, inflammatory cytokines, heme, etc. Transcription of HMOX1 in tissue has been shown to be a product of the nrf2/ARE pathway where an increase in its protein levels results in heme degradation. The tissue protective effects of HMOX1 are a result of the bioactive products produced after heme degradation: iron ions, carbon monoxide, and biliverdin which is converted to bilirubin via biliverdin reductase.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 - 0.5 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.