H2A.X is a 14 kD basal histone and a member of the H2 histone family. This nuclear protein is synthesized in the G1 and S phase of the cell cycle and is known to be important for DNA repair and maintaining genomic stability and for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 after double-stranded DNA breaks. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting, immunofluorescence and flow cytometry.
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.
References
Jha JC, et al. 2013. J. Virol. 87:5255. (FC) PubMed
Akbay A, et al. 2008. Am J Pathol. 173:536. (IHC) PubMed
Mochizuki K, et al.2008.J cell Sci.121:2148. (IF) PubMed
Xiao R, et al. 2007. Mol Cell Biol.27:5393. (IF) PubMed
Rossi DJ, et al. 2007. Nature. 447:725. (IF) PubMed