PE anti-mouse CD273 (B7-DC, PD-L2)

Antibodies Single
Sony
TY25
Flow Cytometry
Rat IgG2a, κ
Mouse
Mouse B7-DC transfected cell line
1136025
$128.00

Description

B7-DC is also called programmed death ligand 2 (PDL2). It has recently been clustered as CD273. This ligand is a 42 kD member of the immunoglobulin receptor superfamily expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions. The TY25 antibody has been reported to be useful for blocking PD-1 mediated interactions.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 microg per million cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Yamazaki T, et al. 2002. J. Immunol. 169:5538. (FC, IP, WB)
2. Ansari MJI, et al. 2003. J. Exp. Med. 198:63. (Block)
3. Salama AD, et al. 2003. J. Exp. Med. 198:71. (IHC)
4. Matsumoto K, et al. 2004. J. Immunol. 172:2530. (FC, Block)
5. Yamazaki T, et al. 2005. J. Immunol. 175:1586. (Block)
6. Meng Q, et al. 2006. Invest Opthalmol Vis Sci. 47:444. (WB) PubMed
7. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
8. Sugita S, et al. 2013. Invest Ophthalmol Vis Sci. 54:3240. PubMed
9. Wong TW, et al. 2014. J. Immunol. 192:3548. PubMed
10. Ochiai S, et al. 2014. J Immunol. 193:2504. PubMed