Description

T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR γ/δ belongs to the immunoglobulin superfamily, which is involved in the recognition of certain bacterial and tumor antigens bound to MHC class I. γ/δ TCR associates with CD3 and is expressed on a T cell subset found in the thymus, the intestinal epithelium, and the peripheral lymphoid tissues and peritoneum. Most γ/δ T cells are CD4^-/CD8^- although some are CD8⁺. T cells expressing the γ/δ TCR have been shown to play a role in oral tolerance, tumor-associated tolerance, and autoimmune disease. It has been reported that γ/δ T cells also play a principal role in antigen presentation.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Goodman T, et al. 1989. J. Exp. Med. 170:1569. (FC, IP)
2. Cardona AE, et al. 2003. Infect. Immun. 71:2634. (IHC)
3. Kapp JA, et al. 2004. Immunology 111:155. (Deplete)
4. Skelsey ME, et al. 2001. J. Immunol. 166:4327. (Deplete)
5. Ke Y, et al. 1997. J. Immunol. 158:3610. (Deplete)
6. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
7. Kasten KR, et al. 2010. Infect. Immun. 78:4714. (FC) PubMed
8. Stadanlick JE, et al. 2011. J. Immunol. 187:664. PubMed
9. Van Belle AB, et al. 2012. J. Immunol. 188:462. PubMed