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- Sony Biotechnology
- Sony Biotechnology
CD3: UCHT1 antibody reacts with a combinatorial epitope of CD3ε, a 20 kD chain of the CD3/T-cell receptor (TCR) complex found on all mature T lymphocytes, NK-T cells and some thymocytes.
CD4: RPA-T4 antibody reacts with CD4, a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages.
CD8: RPA-T8 antibody reacts with CD8a, a 32-34 kD type I glycoprotein. CD8a forms a heterodimer or homodimer with CD8b. CD8 also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (express almost exclusively CD8a homodimers).
For flow cytometric staining, the suggested use of this reagent is 20 microL per million cells or 20 microL per 100 microL of whole blood.