C-C chemokine receptor type 8 (CCR8) CD198, is a 41 kD G-protein coupled receptor with 7 transmembrane regions. CCR8 is expressed by a subset of thymocytes, Tregs, NKT and Th2-polarized cells, a subset of macrophages, monocytes, and monocyte-derived dendritic cells. CCR8 mediates chemotaxis toward its ligand CCL1, and is involved in apoptosis of thymocytes.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* KIRAVIA Blue 520™ has an excitation maximum of 495 nm, and a maximum emission of 520 nm.
References
Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim)
Cibotti R, et al. 1997. Immunity 6:245. (Costim)
Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block)
Nishio M, et al. 1996. J. Immunol. 157:4347. (Block)
Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim)
Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim)
Wang W, et al. 2007. J. Immunol. 178:4885. (Costim)
Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim)