Description

CD34 is a highly glycosylated hematopoietic progenitor antigen. Two isoforms of CD34 have been reported to be generated by alternative splicing. This antigen is expressed on hematopoietic progenitors as well as on endothelial cells, brain, and testis. CD34 is thought to function as an adhesion molecule for early hematopoietic progenitors mediating the attachment of stem cells to extracellular matrix or stromal cells. CD34 is phosphorylated on serine residues by PKC.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

References

1. Garlanda C, et al. 1997. Eur. J. Cell Biol. 73:368. (FC)
2. Knowles HJ, et al. 2004. Circ. Res. 95:162. (IHC)
3. Trempus CS, et al. 2003. J. Invest. Dermatol. 120:501.
4. Winding B, et al. 2002. Clin. Cancer Res. 8:1932. (IHC)
5. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
6. Kairaitis LK, et al. 2005. Am. J. Physiol. Renal. Physiol. 288:F198. (IHC, WB)
7. Ao A, et al. 2008. P. Natl. Acad. Sci. USA 105:7821. PubMed
8. Zaynagetdinov R., et al. 2011. J Immunol. 187:5703. PubMed.