PE/Cyanine5 anti-human Siglec-8

Antibodies Single
Sony
7C9
Flow Cytometry
Mouse IgG1, κ
Human
Recombinant Siglec-8 fused to human IgG Fc
2335570
$405.00

Description

Siglec-8 is a lectin specific for 6'-sulfo-sLex and a member of the Ig-superfamily. It is expressed almost exclusively in eosinophils; however, basophils and mast cells can express it to a lower degree. Siglec-8 is a 54 kD transmembranal protein; the extracellular domain has one V-set Ig-like domain and two C2-set domains. The cytoplasmic domain has two immunoreceptor tyrosine-based inhibitor motifs (ITIM) that recruit SH2-family phosphatases after tyrosine phosphorylation. There are reports that siglec-8 inhibits the release of histamine and prostaglandin D2 mediated by the IgEFcR. This molelcule is also involved in the induction of apoptosis.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA)

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

  1. Floyd H, et al. 2000. J. Biol. Chem. 275:861.
  2. Wen T, et al. 2014. J Immunol. 192:5481. PubMed