Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (α_v β₃ integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 711™ excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711™ is a trademark of Sirigen Group Ltd.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

References

1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
8. Chacko AM, et al. 2012. PLoS One 7:e34958.
9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed