Description

Helios is a member of the Ikaros family of zinc finger transcription factors.  It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members.  Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes.  It is found at high levels in a subpopulation of regulatory T cells.  Helios plays an important role in T cell development and homeostasis.  Overexpression of Helios profoundly alters αβ T cell differentiation and activation.  It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function.  Helios is also involved in tumor immunity.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
 

References

1. Thornton AM, et al. 2010. J. Immunol. 184:1. PubMed
2. Verhagen J and Wraith D. 2010. J. Immunol. 185:7129.
3. Stone B, et al. 2012. Clin Immunol. 145:153. PubMed
4. Vaeth M, et al. 2012. PNAS. 109:16258. PubMed
5. Angin M, et al. 2014. PLoS One. 9:86920. PubMed
6. Bedke T, et al. 2014. Immunol Cell Biol. PubMed
7. Liu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:177. PubMed
8. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed