PE anti-human CD40

Antibodies Single
Sony
HB14
Flow Cytometry
Mouse IgG1, κ
Human
2165030
$231.00

Description

CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The HB14 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially block CD40 binding to CD40L and rescue B cells from apoptosis.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Test size products are transitioning from 20 microL to 5 microL per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 microL staining volume or per 100 microL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

References

1. Pound JD, et al. 1999. Int. Immunol. 11:11. (Costim)
2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
3. Armengol MP, et al. 2001. Am. J. Pathol. 159:861.
4. Cavanagh LL, et al. 2005. Arthritis Res. Ther. 7:R230.
5. Jayakumar A, et al. 2008. Infect Immun.76:2138. PubMed
6. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
7. Iwata Y, et al. 2011. Blood. 117:530. PubMed