CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* KIRAVIA Blue 520™ has an excitation maximum of 495 nm, and a maximum emission of 520 nm.
References
Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)