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- Sony Biotechnology
- Sony Biotechnology
CD86 is an 80 kD immunoglobulin superfamily member, also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells and astrocytes. CD86 along with CD80 are the ligands of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. The PO3 antibody has been shown to block autoantibody production in vivo and inhibit T cell activation in vitro.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 microg per 106 cells in 100 microL volume. It is recommended that the reagent be titrated for optimal performance for each application.
1. Nakajima A, et al. 1995. Eur. J. Immunol. 25:3060.
2. Nuriya S, et al. 1996. Int. Immunol. 8:917.
3. Kasprowicz DJ, et al. 2000. J. Immunol. 165:680.
4. Saito K, et al. 1998. J. Immunol. 160:4225.
5. Nakajima A, et al. 1998. J. Immunol. 161:1901.
6. Ma XT, et al. 2006. Cancer Research 66:1169.
7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
8. Bhatnagar S and Schorey JS. 2007. J. Biol. Chem. doi:10.1074/jbc.M702277200.
9. Giroux M, et al. 2007. J. Immunol. 179:4492.