PE/Cyanine7 anti-human CD116
Description
CD116 is a 70-85 kD α chain of the GM-CSF receptor. It combines with CDw131 β chain to form the high affinity GM-CSF receptor. A soluble form of CD116, which binds GM-CSF with a relatively low affinity, has been identified. In addition, an alternatively spliced form of CD116 with an altered cytoplasmic tail has been described. CD116 is expressed on various myeloid cells including monocytes, macrophages, neutrophils, eosinophils, dendritic cells and their precursors, fibroblasts, and endothelial cells. CD116 is expressed on myeloid leukemias, osteogenic sarcoma cell lines, osteoblast-like cells and breast and lung carcinoma cell lines.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
True-Stain Monocyte Blocker™ is needed in order to minimize non-specific staining of monocytes.
References
- Stomski FC, et al. 1998. J. Biol. Chem. 273:1192. (WB)
- McClure B, et al. 2001. Blood 98:3165. (IP)
- Guthridge MA, et al. 2004. Blood 103:820.
- Xiong S, et al. 2013. J. Clin. Invest. 123:4264. (IHC)
- Sawada H, et al. 2014. J. Exp. Med. 211:263. (IHC).