Description

T cell receptor (TCR) Vγ2 bearing T lymphocytes make up a significant proportion of γδ TCR cells in late fetal and adult peripheral lymphoid tissues. TCR γδ T cells may play a role in immunological surveillance for stress-induced self-antigens. The frequency of Vγ2 expression in different strains varied from 12% to 54% in the TCR γδ repertoire. Variations in the levels of Vγ2⁺ cells are not associated with MHC haplotype. High Vγ2 expression is influenced by the TCR-δ locus.  Expanding Vγ2⁺ TCRγδ cells in B6 mice overwhelmingly use a Vδ7⁺ δ chain except in the DBA/2 strain.

Formulation

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

References

1. Dent AL, et al. 1990. Nature 343:714.
2. Kelly KA, et al. 1993. Int. Immunol. 5:331.
3. Sperling AI, et al. 1992. J. Immunol. 149:3200.
4. Sperling AI, et al. 1997. J. Immunol. 159:86.