PE/Dazzle™ 594 anti-human CD162

Primary Antibody
Sony
KPL-1
Flow Cytometry
Mouse IgG1, κ
Human,Non-human primate,Other
PSGL-1 transfected murine 300.19 pre B-cell line
2244100
$310.00

Description

CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kD, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

References

  1. Snapp KR, et al. 1998. Blood 91:154.
  2. Snapp KR, et al. 1998. J. Cell Biol. 142:263.
  3. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  4. Miyamura K, et al. 2011. J. Gen. Virol. 92:287. PubMed
  5. Cheng Q, et al. 2012. Lupus. 21:632. PubMed.