APC anti-BrdU

Antibodies Single
Sony
3D4
Intracellular Flow Cytometry
Mouse IgG1, κ
Iodouridine-conjugated ovalbumin
2420565
$107.00

Description

BrdU is a uridine derivative and a structural analog of thymidine, which can be incorporated into DNA during the S-phase of a cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

References

  1. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573.
  2. Hirota K, et al. 2007. J. Exp. Med. 204:41.
  3. Godebu E, et al. 2008. J. Immunol. 181:1798.
  4. Waskow C, et al. 2008. Nat. Immunol. 9:676.